Protective Effect of Cinnamic Acid on Doxorubicin-Induced Acute Toxicity Based on Glutathione (GSH) Levels
Reactive oxygen species (ROS) play a dual role in physiological and pathological processes. Doxorubicin (DOX), a widely used chemotherapeutic agent, exerts its antitumor effects partly through ROS generation but also causes significant oxidative damage to healthy organs, including the heart and liver. Cinnamic acid (CA), a natural phenolic found in various plants, has demonstrated antioxidant and organ-protective properties in previous studies. This study aimed to evaluate the protective effect of CA against doxorubicin-induced acute toxicity by assessing glutathione (GSH) levels in heart and liver tissues. Mice were divided into normal, negative, positive (vitamin C and E), and CA at varying doses. GSH levels were measured using Ellman’s method on the third day following doxorubicin administration. Doxorubicin significantly decreased GSH levels in both tissues. In contrast, CA treatment increased GSH levels in a dose-dependent manner, with percentage increases in the 240 and 480 mg/kg BW groups are 37.35% and 70.23% in liver tissue, 123.67% and 168.12% in heart tissue, respectively. The 480 mg/kg BW CA dose showed comparable effect in heart tissue and greater effect in liver tissue compared to positive controls. These findings suggest that CA may protect against DOX-induced toxicity by enhancing endogenous antioxidant defense, particularly through GSH preservation.
URI
https://repository.itera.ac.id/depan/submission/SB2509190025
Keyword
Cinnamic acid Doxorubicin Glutathione