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Author
Esther, Pramudita Lamrettauli Hutasoit

Date Published
12 Jun 2026

Advisor
Dr. Sarmoko, S.Farm., M.Sc., Apt.,
apt. Nisa Yulianti Suprahman, S.Farm., M.Sc.,

Subject
Farmasi

Publisher

Psoriasis is a chronic immune-mediated inflammatory skin disease characterized by epidermal hyperproliferation, edema, and increased production of nitric oxide (NO). This study aimed to evaluate the anti inflammatory effect of 1% bitter melon (Momordica charantia L.) fruit extract lotion on ear edema thickness and nitric oxide levels in imiquimod-induced psoriatic mice. Male BALB/c mice were randomly divided into five groups: normal control, negative control with imiquimod only, negative control with lotion base, positive control (clobetasol propionate 0.05%), and treatment group receiving 1% bitter melon lotion. Inflammation was induced by topical application of 5% imiquimod for six consecutive days. Ear thickness was measured daily using a digital caliper and analyzed as area under the curve (AUC). Nitric oxide concentrations in dorsal skin lysates were quantified using the Griess reaction and UV-Vis spectrophotometry. The data were then analyzed statistically. The results demonstrated that 1% bitter melon extract lotion significantly reduced ear edema thickness concentrations compared with untreated psoriatic groups (up to 55%) while nitric oxide (53,98%). Furthermore, no significant difference was observed between the bitter melon lotion and clobetasol groups. These findings indicate that 1% bitter melon fruit extract lotion possesses anti-inflammatory activity and shows potential as a topical anti-psoriatic agent.

URI
https://repository.itera.ac.id/depan/submission/SB2606240060

Keyword
Momordica charantia psoriasis nitric oxide ear edema anti-inflammatory